CCBM Seminar Series

Shankar Subramaniam

Department of Bioengineering
Department of Chemistry & Biochemistry
San Diego Supercomputing Center
University of California at San Diego

Deciphering Intracellular Networks in Mammalian Cells

Cells respond to input by invoking signaling and regulatory networks in a context-dependent manner.  Spatio-temporal measurements of genes, proteins and other molecules after input provide quantitative information that can help in the reconstruction of the cellular networks that link input to response.  In the Alliance for Cellular Signaling project, primary cells were subject to varied input of distinctive ligands singly and in combination to study signal transduction response.  The response was quantitatively monitored through independent measurements of free intracellular calcium, cAMP, a few proteins and their interactions and several genes as a function of time.  In this talk, I will present the challenges faced in combining and analyzing heterogeneous data from intracellular measurements in reconstructing cellular networks in a context-specific manner. Specifically, I will discuss how the use of biological knowledge in combination with the cellular data can lead to a systemic picture of the cellular response.

Note: This seminar series is videoconferenced to Talbot library, Traylor building . For information on videoconference bridging, or on disability access, contact Sunghee Flores at 410-516-4116 or sflores@bme.jhu.edu

Friday
September 12, 2003
4:00-5:00pm

Room 110,
Clark hall

Video Archive
[ 300kbps ] Real